Spin Doctor Genomic Solution Set

Spin Doctor Genomic Solutiom Set

Fast, Easy, Phenol Free, Economical Tail Prep

Procedure:

1. This kit requires you to supply the Proteinase K tubes to digest samples in 1-2 hours
2. After digest, use the Spin Doctor Genomic Wash Buffer to clean up the DNA
3. Produces great yields of very clean DNA without tedious protocols or hazardous solvents

Inexpensive: Produces clean DNA elinimating the need for hot start taq!

Fast Cleanup Protocol: 15 min. for PCR quality
30 min. for Southern Blotting quality

Great Yield: 40 – 60 ug per sample

Format: Individual 2.0mL tubes

Sample Origination: rodent tail, ear punch or cultured cells

Throughput: 1 – 24 samples (only limited by heat block and centrifuge capacity)

ECOR1 Digest	PCR Genotyping
Agarose gel analysis of genomic DNA from 3 distinct mouse tail samples (1 millimeter section) prepared with the Spin Dr. Genomic DNA Isolation Kit. 15uL of sample was digested with 3 uL of ECO R1 and run on a 1% gel.	PCR analysis of 1 uL samples of 3 distinct mouse tails using both GAPDH and Wild Type Primers.

Protocol

Solution Set Protocol (PDF)

MSDS